{"id":190,"date":"2005-12-22T02:21:01","date_gmt":"2005-12-22T02:21:01","guid":{"rendered":"https:\/\/updates.xephalon.net\/?p=190"},"modified":"2005-12-22T02:21:01","modified_gmt":"2005-12-22T02:21:01","slug":"box-checked","status":"publish","type":"post","link":"https:\/\/www.xephalon.net\/updates\/2005\/12\/22\/box-checked\/","title":{"rendered":"Box checked"},"content":{"rendered":"<p><a href=\"https:\/\/updates.xephalon.net\/wp-content\/uploads\/2005\/12\/2005.12.21.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-thumbnail wp-image-867\" title=\"2005.12.21\" src=\"https:\/\/updates.xephalon.net\/wp-content\/uploads\/2005\/12\/2005.12.21-100x100.jpg\" alt=\"\" width=\"100\" height=\"100\" \/><\/a>After all the primers, PCRs, digests, agarose gels, gel extractions, precipitations, ligations, transformations, colonies, innoculations, incubations, minipreps, midipreps, and maxipreps; the in vitro transcriptions, dig labeling, prehybs, hybridizations, washes, and development; cell lysis, denaturations, quantifications, polyacrylamide gels, coomassies, transfers, blocks, primaries, secondaries, ECL, films, and casettes; after filtering, autoclaving, thawing, splitting, counting, freezing, plating, transfections, cultures, and co-cultures; the co-IPs, protein purifications, equilibrating, loading, washing, eluting, and dialyzing; the superinfections, harvesting, concentrations, and titering, the perfusions, fixations, cryoprotections, cryosections, permeabilizations, and immunostaining, the dissections, triturations, immunopanning, purifications, counting, coating, plating, and replating; after the surgeries, anesthesias, incisions, sutures, injections, implantations, electroporations, hemisections, transections, and crushes; after making buffers, washing tools, and aliquoting solutions; the rinsing, washing, vortexing, and spinning &#8230; the box has finally been <a href=\"updates\/data\/images\/2005.12.21.jpg\">checked<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>After all the primers, PCRs, digests, agarose gels, gel extractions, precipitations, ligations, transformations, colonies, innoculations, incubations, minipreps, midipreps, and maxipreps; the in vitro transcriptions, dig labeling, prehybs, hybridizations, washes, and development; cell lysis, denaturations, quantifications, polyacrylamide gels, coomassies, transfers, blocks, &hellip; <a href=\"https:\/\/www.xephalon.net\/updates\/2005\/12\/22\/box-checked\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-190","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/posts\/190","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/comments?post=190"}],"version-history":[{"count":0,"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/posts\/190\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/media?parent=190"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/categories?post=190"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.xephalon.net\/updates\/wp-json\/wp\/v2\/tags?post=190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}