After all the primers, PCRs, digests, agarose gels, gel extractions, precipitations, ligations, transformations, colonies, innoculations, incubations, minipreps, midipreps, and maxipreps; the in vitro transcriptions, dig labeling, prehybs, hybridizations, washes, and development; cell lysis, denaturations, quantifications, polyacrylamide gels, coomassies, transfers, blocks, primaries, secondaries, ECL, films, and casettes; after filtering, autoclaving, thawing, splitting, counting, freezing, plating, transfections, cultures, and co-cultures; the co-IPs, protein purifications, equilibrating, loading, washing, eluting, and dialyzing; the superinfections, harvesting, concentrations, and titering, the perfusions, fixations, cryoprotections, cryosections, permeabilizations, and immunostaining, the dissections, triturations, immunopanning, purifications, counting, coating, plating, and replating; after the surgeries, anesthesias, incisions, sutures, injections, implantations, electroporations, hemisections, transections, and crushes; after making buffers, washing tools, and aliquoting solutions; the rinsing, washing, vortexing, and spinning … the box has finally been checked.